When an agar plate is inoculated, why is the loop sterilized after the initial inoculum is put on?Create a 2- to 3-page document in Microsoft Word for providing answers to questions in the following review sheets:Support your responses with examples.Cite any sources in APA format.Exercise 4: Pure bacterial colonies1. When an agar plate is inoculated, why is the loop sterilized after the initial inoculum is put on?2. Distinguish between a pure culture and a mixed culture.3. Define a bacterial colony. List four characteristics by which bacterial colonies may be distinguished.4.Why should a Petri dish not be left open for any extended period?5. Why does the streaking method you used to inoculate your plates result in isolated colonies?